Chromaffin cell cultures and secretion assays

Chromaffin cell cultures were prepared from bovine adrenal glands by collagenase digestion and further separated from debris and erythrocytes by centrifugation on Percoll gradients as described ^{Reference 8-10}.

Briefly, cells were maintained in monolayer cultures at a density of 625,000 cells/cm² and were used between the third and sixth day after plating. All the experiments were performed at 37⁰C.

Secreted [³H]noradrenaline was determined in digitonin-permeabilized cells as described ^{Reference 8-10}.

The CPM released from control cells under basal conditions were ~ 3,000, and they were increased to ~ 11,000, when stimulated with 10 µM Ca²⁺.

The total number of counts obtained from detergent-permeabilized cells was ~ 110,000.

Thus, the normalized basal release represents the 3.5% of the total secretion, and the Ca²⁺ -evoked ~ 10%.

Statistical significance was calculated using Student t-test with data from 4 or more independent experiments.

For further clinical data on Argireline^®

• Summary of the clinical trials done on Argireline
• The effect it has on frowns marks and wrinkles
• The anti-wrinkle and anti-aging effect
• Results of the anti-wrinkle test done on humans
• Chromaffin cell cultures and secretion assays
• Synthesis and purification of the Peptide
• Clinical references
• Recombinant SNARE proteins expression and purification

The anti-wrinkle and anti-aging properties of this hexapeptide

At a 10% inclusion rate of this non-toxic, non-irritant hexapeptide molecule, is was noted that it modulates the excessive stimulation of the facial muscles, relaxing facial tension and reduces the depth of already formed wrinkles.

Active ingredients used in Créme de Vie

• Argireline®
  • Clinical studies on Argireline^®
• Hydromanil®
  • Clinical studies on Hydromanil^®
• Vitamin E
• Aldenine®
  • Clinical studies on Aldenine®
• Hyaluronic acid
  • Clinical studies on Hyaluronic acid
• Cranberry oil
• Avocado oil
• Jojoba oil
  • Clinical studies on Jojoba oil
• Rosehip oil
• Lime essential oil
• Full list of ingredients

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