Summary of the anti-wrinkle and anti-aging effect of the hexapeptide

Botulinum neurotoxins (BoNTs) represent a revolution in cosmetic science because of their remarkable and long-lasting anti-wrinkle activity.  However, their high neurotoxicity seriously limits their use. Thus, there is a need to design and validate non-toxic molecules that mimic the action of BoNTs.

The hexapeptide Ac-EEMQRR-NH₂ (Argireline®) was identified as a result of a rational design program.

Noteworthy, skin topography analysis of an oil/water (O/W) emulsion containing 10% of this hexapeptide solution on healthy women volunteers reduced wrinkle's depth up to 30% upon 30 days treatment.

Analysis of the mechanism of action showed that the hexapeptide significantly inhibited neurotransmitter release with a potency similar to that of BoNT A, although, as expected, it displayed much lower efficacy than the neurotoxin.

Inhibition of neurotransmitter release was due to the interference of the hexapeptide with the formation and/or stability of the protein complex that is required to drive Ca ²⁺ -dependent exocytosis, namely the vesicular fusion (known as SNARE) complex.

Notably, the hexapeptide did not exhibit in vivo oral toxicity nor primary irritation at high doses.

Taken together, these findings demonstrate that this hexapeptide is a non-toxic, anti-wrinkle molecule that emulates the action of currently used BoNTs. Therefore, the inclusion of this hexapeptide included at 10% represents a biosafe alternative to BoNTs in cosmetics.

To order your Créme de Vie, which contains this new molecule at the correct 10% inclusion factor, please click here

Underneath the clinical results of skin topography using silicon impressions taken from the lateral preorbital area in healthy women volunteers and analyzed by confocal microscopy.

Reduction of wrinkle depth

The top row illustrates the use of a placebo cream, while the bottom row illustrates the use of the molecule at a 10% inclusion rate. The three periods from left to right illustrates the measurements taken at day 0, day 15 and day 30.

For further clinical data on Argireline^®

• Summary of the clinical trials done on Argireline
• The effect it has on frowns marks and wrinkles
• The anti-wrinkle and anti-aging effect
• Results of the anti-wrinkle test done on humans
• Chromaffin cell cultures and secretion assays
• Synthesis and purification of the Peptide
• Clinical references
• Recombinant SNARE proteins expression and purification

The anti-wrinkle and anti-aging properties of this hexapeptide

At a 10% inclusion rate of this non-toxic, non-irritant hexapeptide molecule, it was noted that it modulates the excessive stimulation of the facial muscles, relaxing facial tension and also reduces the depth of already formed wrinkles.

Active ingredients used in Créme de Vie

• Argireline®
  • Clinical studies on Argireline^®
• Hydromanil®
  • Clinical studies on Hydromanil^®
• Vitamin E
• Aldenine®
  • Clinical studies on Aldenine®
• Hyaluronic acid
  • Clinical studies on Hyaluronic acid
• Cranberry oil
• Avocado oil
• Jojoba oil
  • Clinical studies on Jojoba oil
• Rosehip oil
• Lime essential oil
• Full list of ingredients

Articles of interest

• How bad skincare will impact on the way you look
• Nutrition and your skin and how it can impact on your looks
• The skin - its construction and function
• Cigarette smoking and skin aging
• How wrinkles are formed
• Effect of prolonged illness on your skin
• Sun damage to the skin
• Botulism and Clostridium botulinum
• Information on Botox injections
• Benefits of our anti-aging and anti-wrinkle cream
• Unethical cosmetic manufacturers
• Search engine
• Shopping cart security
• Privacy statement
• Currency converter